Measurement

Part:BBa_K2406061

Designed by: Jack T. Suitor   Group: iGEM17_Edinburgh_UG   (2017-10-07)


Slox-Term-Lox Measurement Construct

This part was designed to test if Slox and Lox Target sites were orthogonal. If either Cre or SCre recombinase could recognise both sites, it would catalyse recombination leading to expression of RFP. If the target sites were orthogonal, no RFP would be produced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 888
    Illegal AgeI site found at 1000
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None